mInDel: an efficient pipeline for high-throughput InDel marker discovery
نویسندگان
چکیده
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منابع مشابه
Genome-wide insertion–deletion (InDel) marker discovery and genotyping for genomics-assisted breeding applications in chickpea
We developed 21,499 genome-wide insertion-deletion (InDel) markers (2- to 54-bp in silico fragment length polymorphism) by comparing the genomic sequences of four (desi, kabuli and wild C. reticulatum) chickpea [Cicer arietinum (L.)] accessions. InDel markers showing 2- to 6-bp fragment length polymorphism among accessions were abundant (76.8%) in the chickpea genome. The physically mapped 7,64...
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With the advent of next generation sequencing (NGS) technologies, single nucleotide polymorphisms (SNPs) have become the major type of marker for genotyping in many crops. However, the availability of SNP markers for important traits of bread wheat (Triticum aestivum L.) that can be effectively used in marker-assisted selection (MAS) is still limited and SNP assays for MAS are usually uniplex. ...
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Motivation: We consider the correction of errors from nucleotide sequences produced by next-generation sequencing. The error rate in reads has been increasing with the shift of focus of mainstream sequencers from accuracy to throughput. Denoising in high-throughput sequencing is thus becoming a crucial component for boosting the reliability of downstream analyses. Results: Our methodology, name...
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Development and large-scale genotyping of user-friendly informative genome/gene-derived InDel markers in natural and mapping populations is vital for accelerating genomics-assisted breeding applications of chickpea with minimal resource expenses. The present investigation employed a high-throughput whole genome next-generation resequencing strategy in low and high pod number parental accessions...
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تاریخ انتشار 2014